The formula and calculation is a starting point to get into the area of steps per inch. You will then need to use the mach3 calibration utility to get the exact steps per inch.
Formula:
step per inch = (motor steps * microstepping) / (travel at one turn of the motor in inches)
if microstepping is set at 16 (1/16 on the driver) then and you are using a sprocket and chain with a pitch of .25 inches and 12 teeth on the drive sprocket
= (200 * 16) / (12 * .25)
= 3200 / 3
= 1066.666 steps per inch
For lead screw that has a travel of .5 inches at one turn like the 5 start 1/2 inch lead screw and using 4 microsteps per step (1/4):
= (200 * 4) / (.5)
= 800 / .5
= 1600 steps per inch
Remember to use the mach3 calibration wizard and double check the driver microstep setting.
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WHAT IS THE FORMULA TO DETERMINE STEPS PER INCH OR RESOLUTION FOR EACH AXIS?